| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.024214 |
| Chromosome: | chromosome 6 |
| Location: | 4090086 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278226 | (1 of 6) PTHR31942//PTHR31942:SF1 - FAMILY NOT NAMED // MLO-LIKE PROTEIN 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGAGCGCGAAGCCCACGAAGATGACTGACAGGAACAGGAAGAACAGCA |
| Internal bar code: | AGGAGCGAGTGATGTTCGGATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2412 |
| LEAP-Seq percent confirming: | 90.9091 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTTTTGGAGCGAGGGAGG |
| Suggested primer 2: | GTCTCGCTCTCCACGATCAG |