Insertion junction: CLIP2.024235_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.024235
Chromosome:chromosome 12
Location:3116587
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g500850 (1 of 85) IPR009057 - Homeodomain-like CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTGTCCAAGTTGGATGACGTGCGCGCTATGCTCATCTCACGGCGTAGCC
Internal bar code:TGCGTAAATGTGATATGCCATT

Confirmation - LEAP-Seq

LEAP-Seq distance:6421
LEAP-Seq percent confirming:97.5904
LEAP-Seq n confirming:81
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:83

Suggested primers for confirmation by PCR

Suggested primer 1:GCAGTTCTATTGCCAGCAGC
Suggested primer 2:GAACCACACACTGCGAAACC