Insertion junction: CLIP2.024237_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.024237
Chromosome:chromosome 1
Location:5719896
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g040950 (1 of 1) PF02816//PF13519 - Alpha-kinase family (Alpha_kinase) // von Willebrand factor type A domain (VWA_2) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTTGCATCCATTCTCAGTATGCAACCAACTTTCCCCACCAGGAGTACGA
Internal bar code:GGGACAATGTAAATTCATTCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:2067
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:39
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:39

Suggested primers for confirmation by PCR

Suggested primer 1:TGCGTGTACCCGAATCAACA
Suggested primer 2:CATGCACGACCGTTTGTTGT