| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.024261 |
| Chromosome: | chromosome 3 |
| Location: | 3447549 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g166900 | CYP767A1,CYP15 | (1 of 1) 1.14.13.98 - Cholesterol 24-hydroxylase / Cytochrome P450 46A1; Cytochrome P450, CYP4 superfamily | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGATGCCGCGTGTGCCCGCCGTCTGTAGCGTGAGGTGCGTGCTCTCGC |
| Internal bar code: | TTATATTTTGGCTCAGAGAATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 308 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGAGGTCATGACGGTGTTG |
| Suggested primer 2: | CAGTCCACGCACCAAAAAGG |