Insertion junction: CLIP2.024271_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.024271
Chromosome:chromosome 10
Location:499123
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g421050 (1 of 9) 2.7.10.1 - Receptor protein-tyrosine kinase / Receptor protein tyrosine kinase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGATGGGCCCTAGTGCTGCTGCTGCTGCTGTCCATCAGAGTAGCGCCACC
Internal bar code:ATACTATTGAAAGATTGTGTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:2390
LEAP-Seq percent confirming:98.4615
LEAP-Seq n confirming:64
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:65

Suggested primers for confirmation by PCR

Suggested primer 1:GACAGCAGATTGCCAGAGGT
Suggested primer 2:CGCGTAGAAGGCACTCTCAT