Insertion junction: CLIP2.024288_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.024288
Chromosome:chromosome 2
Location:6541738
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g389089 CNX5,NOFNIR1,NOFNiR,ARC1 NO-forming nitrite reductase; (1 of 1) PTHR14237:SF30 - MITOCHONDRIAL AMIDOXIME-REDUCING COMPONENT 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTAAATTTGCGCACCCGCGCCGTCTGTCGCGCATGCCCGCAACTCCGCA
Internal bar code:CCGGCCCATTTACAATGTCTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:2604
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:23
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGCAGCTGGTATCGATCT
Suggested primer 2:TGTTGTCATCATCGTCCGCA