| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.024300 |
| Chromosome: | chromosome 3 |
| Location: | 5810370 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g187950 | SEE1 | Putative splicing endonuclease positive effector; (1 of 3) K10706 - senataxin [EC:3.6.4.-] (SETX, ALS4) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGACGCAGAGGGTGGGCCACAAGGGCCAGGCACCGCGCCGCCGCCCAAT |
| Internal bar code: | TCCTTCATTCGTATGTGAGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2880 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGGCAGCTGAAATTCTCT |
| Suggested primer 2: | CAGCTGTTGGTGTGCTTGTC |