| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.024318 |
| Chromosome: | chromosome 4 |
| Location: | 1069104 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g216350 | AKR7,CPLD3 | Conserved in the Plant Lineage and Diatoms; (1 of 1) K05275 - pyridoxine 4-dehydrogenase (E1.1.1.65) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAATGTACCGATGTGATCTGCGTTGTACGGTGGGCTGGGTTGTCCCCAC |
| Internal bar code: | AAGATGTCTGCGAAATACAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1891 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTGCAACCCCATTCCTAC |
| Suggested primer 2: | TGTGTACAGTCTGCTGCAGG |