| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.024320 |
| Chromosome: | chromosome 6 |
| Location: | 3149779 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g275850 | HTA6,HTA3 | Histone H2A; (1 of 30) K11251 - histone H2A (H2A) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAGAACCCCTAACTCGATACAATGGCTGGTCGCGGCAAGGGCAAGACCT |
| Internal bar code: | AATTCGTAAAAGGGAACTAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4576 |
| LEAP-Seq percent confirming: | 95.4545 |
| LEAP-Seq n confirming: | 126 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 132 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACCAAACCCGTTCCCAAA |
| Suggested primer 2: | GGTCCAGCCTGAGAGCTTTT |