Insertion junction: CLIP2.024334_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.024334
Chromosome:chromosome 3
Location:348038
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g144607 (1 of 79) IPR011992 - EF-hand domain pair intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCTCAACGGGACTACCCAGCAACCACACAAGGCTTGCATTTCATTAGTC
Internal bar code:GTGCTTCGTGTCGCCGCGTGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:102
LEAP-Seq percent confirming:62.5
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:ACCTTTCACCACAGCACACA
Suggested primer 2:TTGTTGATTACCGTGGGCGA