Insertion junction: CLIP2.024347_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.024347
Chromosome:chromosome 7
Location:3541505
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g336400 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCAGATGCCTCAGGCGCCCAGACGCCCACCCACCCGCGGCGCCTGCTTG
Internal bar code:TTTGGGTACGCATGTCGTTTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:2183
LEAP-Seq percent confirming:76.9231
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:CCTCGATCCTCCGTCATTCG
Suggested primer 2:GTGGAATGGTGTGGCGAAAC