Insertion junction: CLIP2.024359_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.024359
Chromosome:chromosome 2
Location:1606521
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g085050 COP1,PCOP1 Plant-type constitutive photomorphogenesis protein 1; (1 of 2) K10143 - E3 ubiquitin-protein ligase RFWD2 (RFWD2, COP1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCAGTTGGACGCCCACCAACGTGCGTGTGAGACGTTCCGTTATTCCACA
Internal bar code:TTCGGCTTACATAGATGATGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:2427
LEAP-Seq percent confirming:81.25
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:CCCCGCATATTTCAGCATGC
Suggested primer 2:GCGTCACACCATATGTTCGC