Insertion junction: CLIP2.024385_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.024385
Chromosome:chromosome 7
Location:4895973
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g346500 (1 of 7) IPR000104//IPR020683 - Antifreeze protein, type I // Ankyrin repeat-containing domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTGACGAGGCTGCGGTGCGGTGGCTGCTGTTTGAGGCGGGTGTGGAACT
Internal bar code:TGGGTACGGGTCGGTGCACGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1381
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GGGGGAAATAGTGGTTGGGG
Suggested primer 2:ATCTGTATCGCACCGGACAC