| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.024394 |
| Chromosome: | chromosome 12 |
| Location: | 709545 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g492851 | EBM7 | (1 of 4) K19355 - mannan endo-1,4-beta-mannosidase (MAN); Mannan endo-1%252C4-beta-mannosidase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTATGACACAAGGATCAGGCGCTGCTGATGCAGCGGGGTCCCCGGCAT |
| Internal bar code: | TGGCGGAAACTGCCAGGGTTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5212 |
| LEAP-Seq percent confirming: | 98.8372 |
| LEAP-Seq n confirming: | 85 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 86 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTTCTTGCATGCGAGTACG |
| Suggested primer 2: | AGCACCTAGTGATGTACGCG |