| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.024403 |
| Chromosome: | chromosome 9 |
| Location: | 2159409 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g395650 | (1 of 2) PF13806 - Rieske-like [2Fe-2S] domain (Rieske_2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACCAGCATCGCTCGCACCCGTCGCACCGCCGCCACCGCTGCCGCGGCG |
| Internal bar code: | TTATGCGATCGGGGTTGGTTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2349 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 73 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 73 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTGTTCGCCTATTTCGTG |
| Suggested primer 2: | CGCAAGGATGTTTGTGTGCA |