| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.024406 |
| Chromosome: | chromosome 12 |
| Location: | 1554545 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g487600 | CPS2 | Putative cleavage and polyadenylation specificity factor 100 kDa subunit; (1 of 1) K14402 - cleavage and polyadenylation specificity factor subunit 2 (CPSF2, CFT2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTACTTGCAAGGGATGGGTGGGTCGGCGCCGGTGCTTAAGGGCATGAC |
| Internal bar code: | ACAGGACCAGAATATTGGCTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1004 |
| LEAP-Seq percent confirming: | 80.7692 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCATAGATGACTTCCCGA |
| Suggested primer 2: | TGCTTTCCACTTTGCAACCG |