Insertion junction: CLIP2.024411_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.024411
Chromosome:chromosome 11
Location:3709277
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g801327 (1 of 70) PF07282 - Putative transposase DNA-binding domain (OrfB_Zn_ribbon) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAACCTAATTGCTGCGAGCGTTGCCTCGTCCTTGCAAACGCTTCGCAGCG
Internal bar code:ACAATATCTTTCTATTGTCGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1492
LEAP-Seq percent confirming:68.75
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:ACGTACCCTGCTTGGACATG
Suggested primer 2:GGTGAGGACGTCTTGAAGCA