Insertion junction: CLIP2.024424_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.024424
Chromosome:chromosome 16
Location:230019
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g694704 (1 of 68) 2.1.1.43 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCACACGCCACGGCGCGCCATCAGCACGAACCCCTCCCCCTTCAGGCGGT
Internal bar code:CCTTTTTGGACTAGGATAACTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1916
LEAP-Seq percent confirming:90.4762
LEAP-Seq n confirming:19
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:CTGTCCAGGGCTCTTCACAG
Suggested primer 2:CAGTCCATAGCTAGCCACGG