| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.024442 |
| Chromosome: | chromosome 7 |
| Location: | 3566689 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g336650 | MLH3 | DNA mismatch repair protein; (1 of 1) IPR003594//IPR020568 - Histidine kinase-like ATPase, C-terminal domain // Ribosomal protein S5 domain 2-type fold | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACGGACCGCTGCGTGGGCTGCTGCGCTTGCAGTCTACGGGCCGGGCTGC |
| Internal bar code: | CTGCGGAGAAAATGGGAATTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2223 |
| LEAP-Seq percent confirming: | 88.4615 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCATCTCCAGGGCTGACT |
| Suggested primer 2: | TGCTCAACGCCCACTTTTTG |