| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.024451 |
| Chromosome: | chromosome 12 |
| Location: | 3281056 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g499200 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAAGTATTACTCAGGGGTCCGTGCTTGGGGCAAAGCCCATGCTGACAT |
| Internal bar code: | AGTGCGCATAGTGAAGGGACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3210 |
| LEAP-Seq percent confirming: | 76.7442 |
| LEAP-Seq n confirming: | 66 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 86 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTGAGAGGGTTGAATGGG |
| Suggested primer 2: | CGGGAGGACGACTTATTCCG |