Insertion junction: CLIP2.024455_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.024455
Chromosome:chromosome 9
Location:2984558
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g393395 (1 of 1) PTHR33477:SF3 - EMB|CAB72147.1 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGACGTAAGCGGCACTGCCGCACCTGTCAGTGGCGGTGCCGGCACGGTTG
Internal bar code:GTAAATACACGTTGGGGTTCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1601
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:23
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:CACTTCATCGGAGCAGTGGT
Suggested primer 2:GCTGCTTCGGCCATAGCTAT