| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.024458 |
| Chromosome: | chromosome 9 |
| Location: | 136305 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g386700 | kinesin-13,KIN13-1,CrKin13,KIN13A | Kinesin motor protein 13; (1 of 1) K10393 - kinesin family member 2/24 (KIF2_24, MCAK) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACGAGGGCTAGGCAGGCCTGTGCCCGAGCCCTATAACTCATCCAGGCGT |
| Internal bar code: | AGCACCCTAAGTGCGGAGACCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4446 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATCTACTGGCCGACGTGAG |
| Suggested primer 2: | AGCAAGTCGAACACCTGAGG |