| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.024490 |
| Chromosome: | chromosome 13 |
| Location: | 3139830 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g584850 | (1 of 15) 2.5.1.18 - Glutathione transferase / S-(hydroxyalkyl)glutathione lyase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATCTGGGTGCCCCTGCTCGCGACGAGTCAGGAGAACAGCGGAAGTGAGC |
| Internal bar code: | CCAGTTAGACAAGTAAATCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3162 |
| LEAP-Seq percent confirming: | 91.1765 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAATGTTGTTTGGCTCGGT |
| Suggested primer 2: | AAGAGGGGCAGGTAGAGGAG |