Insertion junction: CLIP2.024505_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.024505
Chromosome:chromosome 7
Location:2011951
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g325761 (1 of 2) IPR000104//IPR003439//IPR003593//IPR011527//IPR027417 - Antifreeze protein, type I // ABC transporter-like // AAA+ ATPase domain // ABC transporter type 1, transmembrane domain // P-loop containing nucleoside triphosphate hydrolase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGGACGCCGCAACCTCCATCCACAACCTCATGGCAAAGAGGTGAGCCGG
Internal bar code:AAGCATATGGGCGCTCAGGTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1109
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:AACTCAGTCAAGGAGTGGCG
Suggested primer 2:TAGTGTGTTGTGCGCTTTGC