Insertion junction: CLIP2.024511_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.024511
Chromosome:chromosome 1
Location:4468679
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g030550 UBX1 (1 of 1) PTHR13020:SF34 - UBX DOMAIN-CONTAINING PROTEIN 4; possible ubiquitin-proteasome pathway protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGAAGGCCTCCTCGCCGCTGCTGCCGGCCACACCCGCCATGTGCGCCTT
Internal bar code:CGGGACGAGAATGTTAGGCATG

Confirmation - LEAP-Seq

LEAP-Seq distance:1898
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:86
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:86

Suggested primers for confirmation by PCR

Suggested primer 1:CCGCTAACCTATCCGTGACC
Suggested primer 2:AATCGGATGACGTGCACACT