| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.024511 |
| Chromosome: | chromosome 1 |
| Location: | 4468691 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g030550 | UBX1 | (1 of 1) PTHR13020:SF34 - UBX DOMAIN-CONTAINING PROTEIN 4; possible ubiquitin-proteasome pathway protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACCATGCTCAAGTGAGCGGGCGACCGGCCGGTGTTGTGCCTGTGGCAGT |
| Internal bar code: | CGGGACGAGAATGTTAGGCATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1393 |
| LEAP-Seq percent confirming: | 95.0 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATCGGATGACGTGCACACT |
| Suggested primer 2: | CCGCTAACCTATCCGTGACC |