Insertion junction: CLIP2.024517_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.024517
Chromosome:chromosome 12
Location:25485
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g483500 (1 of 1) PF06101//PF12624//PF16908//PF16910 - Plant protein of unknown function (DUF946) (DUF946) // N-terminal region of Chorein or VPS13 (Chorein_N) // Vacuolar sorting-associated protein 13, N-terminal (VPS13) // Repeating coiled region of VPS13 (VPS13_mid_rpt) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGCGCAGTACGGCGCACTGGCGGAGGAGTCACCGTACAGGACCATATGC
Internal bar code:ACTAAGTAAGACTGTAGACGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:3250
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:41
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:41

Suggested primers for confirmation by PCR

Suggested primer 1:AGGAGGAAACAGCCAGAAGC
Suggested primer 2:GCGCAGTTCAGCTTCATCAG