Insertion junction: CLIP2.024517_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.024517
Chromosome:chromosome 12
Location:25493
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g483500 (1 of 1) PF06101//PF12624//PF16908//PF16910 - Plant protein of unknown function (DUF946) (DUF946) // N-terminal region of Chorein or VPS13 (Chorein_N) // Vacuolar sorting-associated protein 13, N-terminal (VPS13) // Repeating coiled region of VPS13 (VPS13_mid_rpt) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCAGGCTGGTGGACAGCTCCAGCGAGGTCGGCCGCACGTCCAGTCGCAA
Internal bar code:TTGGCAAAGCTGCCTTCATGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:2723
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:26
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:GCGCAGTTCAGCTTCATCAG
Suggested primer 2:AGGAGGAAACAGCCAGAAGC