Insertion junction: CLIP2.024522_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.024522
Chromosome:chromosome 11
Location:1778529
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g467900 (1 of 10) PF13578 - Methyltransferase domain (Methyltransf_24) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGACTTATCGTGAGGGTATGTGCGGCCGGAAACTCGGAGGGCACGGGTC
Internal bar code:GGGCTACTACGCTCTTAGCTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1363
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:23
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:ACCCACATCAACGACTGCAT
Suggested primer 2:GCAGCTGCACATTTTGACCA