Insertion cassette: | CIB2 |
Side of cassette: | 3' truncated? |
Strand: | - |
Strain: | CLIP2.024537 |
Chromosome: | chromosome 6 |
Location: | 8364396 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre06.g307650 | (1 of 1) PF12138 - Spherulation-specific family 4 (Spherulin4) | outside_mRNA | |
Cre06.g307700 | (1 of 6) 2.7.7.84 - 2'-5' oligoadenylate synthase | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATGGGCCATCCATGGCAAGCGCGCTCCAGACCAGGCTGGCGGTCGGCA |
Internal bar code: | TGTCAAGGCACGGTCTGTACGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1092 |
LEAP-Seq percent confirming: | 89.3617 |
LEAP-Seq n confirming: | 42 |
LEAP-Seq n nonconfirming: | 5 |
LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCGTGCTGCATACATAGGGT |
Suggested primer 2: | TCCACGATGTCCCAGGATCT |