Insertion junction: CLIP2.024555_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.024555
Chromosome:chromosome 11
Location:3249145
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g476250 (1 of 37) PF13639 - Ring finger domain (zf-RING_2) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGACGCCGCTGCGCGCCACGCCCGTGCACCTGCACGTGCACAACACACA
Internal bar code:GGCCGTCTCGGAGACAACTGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:731
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:CCCACACACACAACACACAC
Suggested primer 2:CCTGGCATTACGATTGTGCG