| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.024559 |
| Chromosome: | chromosome 5 |
| Location: | 478937 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g244050 | Chlamydomonas specific protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGAGCAGGCTGCCCTGAAGGAGGCGCAGGCCGCCCAGGCTGCTCAGGC |
| Internal bar code: | AGAAGTGCGACATTGCGGGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5144 |
| LEAP-Seq percent confirming: | 50.0 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCGTCTTGGCCTGAGC |
| Suggested primer 2: | CGTGGCTGAGGAGAAGCG |