| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.024565 |
| Chromosome: | chromosome 10 |
| Location: | 4535826 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g451400 | NIT4,CNX1B,CNX1E | (1 of 1) 2.10.1.1//2.7.7.75 - Molybdopterin molybdotransferase // Molybdopterin adenylyltransferase; Molybdenum cofactor biosynthesis protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTACAGCTTCTGAGGATGACAAAGGGGTAATCAGGTGTATCCATACAC |
| Internal bar code: | TTGGGTAAGTTATAGTTTGAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3841 |
| LEAP-Seq percent confirming: | 98.1818 |
| LEAP-Seq n confirming: | 54 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGATCATACAGACCTGCCCC |
| Suggested primer 2: | CCTACATCACCACAGGCAGG |