Insertion junction: CLIP2.024582_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.024582
Chromosome:chromosome 16
Location:903889
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g648200 CYP744C1,CYP35 (1 of 3) K01832 - thromboxane-A synthase (TBXAS1, CYP5A); Cytochrome P450, CYP3 superfamily intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTATGGGTGTGCTTGCGCGGGCGAGCTAGTAGAAATGGTCTCGCACCCGC
Internal bar code:TATATTAGTACACCGCATATTC

Confirmation - LEAP-Seq

LEAP-Seq distance:1521
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:32
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:ACAACGGGTGTTTGGTCCTT
Suggested primer 2:ATATGGCGCTCTCTGTCTGC