Insertion junction: CLIP2.024595_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.024595
Chromosome:chromosome 6
Location:1848240
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g263800 SEE2 Putative splicing endonuclease positive effector; (1 of 1) PTHR10887:SF369 - DNA2/NAM7 HELICASE FAMILY PROTEIN intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACCTCGCCGTTGAGCAGTCGGCCGTCGTAGAACTCGGCTGAGGGGAAGG
Internal bar code:CGTTACTAGGTACAACATACGT

Confirmation - LEAP-Seq

LEAP-Seq distance:394
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:ACTGTCGTGTGTGTGTGTGT
Suggested primer 2:AAACATAACCACGCACGCAC