Insertion junction: CLIP2.024632_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.024632
Chromosome:chromosome 1
Location:6500981
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g045903 DGAT1 (1 of 1) K11155 - diacylglycerol O-acyltransferase 1 (DGAT1); Diacylglycerol O-acyltransferase, Type 1 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTCCTGGTGCACCTGGCCTCCACCACCGGCGTCATCGCACTGCCTTGGG
Internal bar code:TAGGAGGTAAACAACAATATTG

Confirmation - LEAP-Seq

LEAP-Seq distance:72
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GGGTAGCTAGGGAAAGGGGA
Suggested primer 2:AGGACTAGGTGAGGCAGGAG