| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.024650 |
| Chromosome: | chromosome 10 |
| Location: | 5126995 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g455350 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGGTCAACAGACTCGAGGGACGAGGCAACCGTGTAAACCGCAGTCACA |
| Internal bar code: | GCTCTGAATGTATGCTTTATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1115 |
| LEAP-Seq percent confirming: | 92.8571 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTGTTTATGCGTGTGTGT |
| Suggested primer 2: | GGAATAGGTGCTGGTGGCTT |