Insertion junction: CLIP2.024663_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.024663
Chromosome:chromosome 14
Location:344499
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g610050 (1 of 1) K15180 - negative elongation factor B (COBRA1, NELFB) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGCCTCGTCCAGCGGGGCGCTGCGTGGCGGGTGTGTGTGTGGGGTGGGGT
Internal bar code:TCTATTAATGGTTGTCGTGCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:525
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ACCCCTATGCCTGCTGTCTA
Suggested primer 2:CCGATCCTCAACGTCCTAGC