| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.024665 |
| Chromosome: | chromosome 4 |
| Location: | 2206302 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g220150 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGTGCGGGGTGAGCGAACGAGTAAGGTTTCGTGCGATGGAACAGGTAG |
| Internal bar code: | GACTCATTTGCCCCGCTCGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 932 |
| LEAP-Seq percent confirming: | 76.9231 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGCGGTGCTGCTGATATAT |
| Suggested primer 2: | CCGACCATTCACTGCTCCTT |