| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.024668 |
| Chromosome: | chromosome 13 |
| Location: | 1899440 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g575776 | (1 of 781) IPR000104 - Antifreeze protein, type I | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGATTGCATGGCTTCCTGCTTAGCTTAGCGCTTTTGGCAGTGCGGAGGC |
| Internal bar code: | TTGAGATTAGCATGCAATAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1506 |
| LEAP-Seq percent confirming: | 97.9592 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGGTGACTGATCGGTGTG |
| Suggested primer 2: | TCTGCCCTCAACAACATCCC |