| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.024696 |
| Chromosome: | chromosome 11 |
| Location: | 277106 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467561 | FAP222 | Flagellar Associated Protein 222 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCGCTCGCGACGTTCCATTGCGCCCACCCCTCTCGACAGAACTCTCTT |
| Internal bar code: | AGGAGGACGCTCGTCGTTTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 319 |
| LEAP-Seq percent confirming: | 55.5556 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACGATAGGCCTACCGTGTG |
| Suggested primer 2: | ACAAGTAGCAACGCACGAGA |