| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.024717 |
| Chromosome: | chromosome 1 |
| Location: | 2895208 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g018000 | RPA12 | DNA-directed RNA polymerase I subunit A12.2; (1 of 1) K03000 - DNA-directed RNA polymerase I subunit RPA12 (RPA12, ZNRD1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGGGTAGAGCGCACCACTGGATTATCGGGGCCAGGTTACGCGGGGACC |
| Internal bar code: | TGTATGCGCCGAAACGTCCTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2456 |
| LEAP-Seq percent confirming: | 93.3333 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTAAGCTGCTCTGCACAA |
| Suggested primer 2: | GATCGGAATACTTCGCGGGT |