| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.024722 |
| Chromosome: | chromosome 12 |
| Location: | 5830690 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g533201 | (1 of 3) PF07452 - CHRD domain (CHRD) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGAACAAATCCCGTCAACGGCGGGACCGCGGCGGCTCCCAGGCCGCCC |
| Internal bar code: | TTTTTATTGATTCGTATCGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 488 |
| LEAP-Seq percent confirming: | 24.3902 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCATGCAAAGAGGTAGCA |
| Suggested primer 2: | GTACACACGCACACACACAC |