Insertion junction: CLIP2.024739_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.024739
Chromosome:chromosome 12
Location:3252385
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g499451 (1 of 1) PTHR14089//PTHR14089:SF2 - CELL CYCLE CONTROL PROTEIN // PRE-MRNA-SPLICING FACTOR CWC2 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATAGGGCCACAGGTGCTGGCGTCGCTTGTGACATATGCACACTGTGCAG
Internal bar code:TGAGTAAACTTTTGTTAGAAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:2028
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:GGCCCCGTCAACAACATCTA
Suggested primer 2:CGCTGTTTTTCCCCTTGGTG