Insertion junction: CLIP2.024742_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.024742
Chromosome:chromosome 14
Location:909915
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g613300 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCATTTTGCTCACTTGCGCAAGGTGCTGATCAAATGATGGCGAACCAGGA
Internal bar code:TACTCGTGGTGTGGTGACGTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:436
LEAP-Seq percent confirming:66.6667
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GCACACACATCAACCTGCAG
Suggested primer 2:CCGTCAACACACGACAAACC