Insertion junction: CLIP2.024747_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.024747
Chromosome:chromosome 7
Location:2894510
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g332500 AXT2 (1 of 3) PTHR10994:SF69 - PROLINE-RICH PROTEIN 18; Arabinose chain extension enzyme for HRGP 2 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTGGCGGCCGGGCACCTTGTAGCCCGCCACGTCGCTGTCGCTGTCGCCG
Internal bar code:AGTCAACGCAAAAGACTGCATA

Confirmation - LEAP-Seq

LEAP-Seq distance:1002
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:35
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:35

Suggested primers for confirmation by PCR

Suggested primer 1:GTCCATCGTGGGGCTTTGTA
Suggested primer 2:CGTCACATACGTAGGAGCCC