Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | - |
Strain: | CLIP2.024760 |
Chromosome: | chromosome 12 |
Location: | 6201325 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre12.g535851 | RPL8 | Cytosolic 80S ribosomal protein L8; (1 of 1) K02938 - large subunit ribosomal protein L8e (RP-L8e, RPL8) | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACCAGTGCGGCGGGCGGCAATGAGACCGACCTTCTGGCCGGGGGGCGCC |
Internal bar code: | CTGTGGTATAAATGTAAGCTAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 3311 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 14 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCCAGTACAGCAACCACGAC |
Suggested primer 2: | AACATGACTCACGCTGCAGA |