| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.024769 |
| Chromosome: | chromosome 7 |
| Location: | 3831212 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g338650 | POLI1,CSC3,POLH1 | Chlamydomonadale-specific family C protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAGGTGGCTTTAGACTCCACCTGTTTGCGCCCCTCCTTGAATCCTGCA |
| Internal bar code: | GGAGCAAGCTTCCGTGTGCTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 623 |
| LEAP-Seq percent confirming: | 94.7368 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCCTACGACGTCTACGAG |
| Suggested primer 2: | TGCGCTATGTGTCGGGATAC |