| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.024774 |
| Chromosome: | chromosome 10 |
| Location: | 3051838 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g441000 | (1 of 1) K17498 - transcription factor SPN1 (SPN1, IWS1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCTTGGCCTCACCCGCGGGATGAGCGCGTGGATGCGCATGGCCTTGTT |
| Internal bar code: | TTTGGCTCGACAGGGCCCCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5428 |
| LEAP-Seq percent confirming: | 86.6667 |
| LEAP-Seq n confirming: | 91 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 105 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACCGGAATGCTTGCATAG |
| Suggested primer 2: | CAGTCCAACACAACAGCTGC |