| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.024797 |
| Chromosome: | chromosome 3 |
| Location: | 6147860 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g190800 | TMPK1,DIV28 | dTMP kinase; (1 of 2) 2.7.4.9 - dTMP kinase / TMPK | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGAGAGGGGGCGAGCGGGCTGGTGGGTGCAAGCCTTTATGGCATTCCG |
| Internal bar code: | GTTGGTAGCGATCATGAGTGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1350 |
| LEAP-Seq percent confirming: | 48.1481 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTCGGAAAGCTTGCAAGGC |
| Suggested primer 2: | CCCACAGCCCCAATCAGATT |